Abstract: To investigate the therapeutic efficacy and underlying mechanisms of cylindrin combined with metronidazole for traumatic oral ulcers, we randomly assigned 36 SD rats to different groups. After successful modeling, the rats received respective drug treatments for 7 days. General conditions of each group were observed and recorded. Pathological changes in oral mucosa were examined via hematoxylin-eosin staining, while plasma levels of IL-1 and IL-6 were detected using ELISA. The results showed that the rats in each group exhibited symptoms after successful modeling, such as hunching and curling, reduced behaviors and lethargy, as well as decreased appetite. After drug intervention, these symptoms improved, for one example, the weight of the high-dose group of combined medication is the closest to the blank control group. It was observed that the oral ulcer wounds in the high-dose group of combined medication showed the best effect by treatment. The result of hematoxylin-eosin staining revealed pathological changes in the epithelial structure of the modeling group, while the high-dose group of combined medication showed the best recovery after treatment. Compared to the cylindrin group and the metronidazole group, the results of ELISA indicated that the expression levels of IL-1 and IL-6 were significantly reduced in both the high and low-dose groups of combined medication (P<0.001). This study demonstrated that the mixed solution of cylindrin and metronidazole suppresses the expression of inflammatory factors IL-1 and IL-6, which contributes a more excellent therapeutic influence than single-agent on traumatic oral ulcers. It is expected to provide a better strategy for the clinical treatment of oral ulcers.
Key words: traumatic oral ulcer; cylindrin; metronidazole solution; combination therapy; inflammatory factor
（Acta Laser Biology Sinica, 2025, 34(6): 551-560）