Abstract: To evaluate the efficacy differences of Portulaca oleracea extract, intense pulsed light, and their combination in treating hormone-dependent dermatitis (HDD) in guinea pigs, and to explore their mechanisms of action, this study selected 30 guinea pigs and randomly divided them into 5 groups (6 per group). One group served as the control, while the remaining four groups developed an HDD model using 0.05% clobetasol propionate ointment. After the model was established, one group was designated as the model group, and the other three groups received three treatments: topical application of Portulaca oleracea extract, 590 nm intense pulsed light therapy and the combination of the two. Changes in skin lesions were directly observed, and histopathological alterations were assessed via haematoxylin-eosin staining. Enzyme-linked immunosorbent assay (ELISA) was employed to measure tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), cysteinyl aspartate specific proteinase-14 (Caspase-14) and loricrin (LOR) expression levels in the lesion tissues. The ELISA results revealed elevated IL-6 and TNF-α expression alongside reduced Caspase-14 and LOR expression in the lesion tissues of guinea pigs in the model group (P<0.05). In addition, IL-6 and TNF-α levels were reduced and Caspase-14 and LOR levels were increased in the three treatment groups, and the effect was more significant in the combination group (P<0.05). The study indicates that Portulaca oleracea extract improves guinea pig HDD through anti-inflammatory effects and skin barrier repair, with synergistic enhancement when combined with intense pulsed light. This study aimed to investigate the synergistic therapeutic effects of Portulaca oleracea extract and intense pulsed light in a guinea pig HDD model and elucidate its potential mechanisms of action, thereby providing a reference for clinical treatment.
Key words: hormone-dependent dermatitis; Portulaca oleracea; intense pulsed light; guinea pig; collaborative therapeutic mechanism
（Acta Laser Biology Sinica, 2025, 34(6): 523-529）