Abstract: To explore the effects of microRNA-128-3p (miR-128-3p) on proliferation and invasion of gestational trophoblast cells by modulating silent information regulator 2 homolog 1(SIRT1)/hypoxia inducible factor-1α (HIF-1α) pathway, the placental tissues were collected from preeclampsia patients and normal full-term pregnant women who gave birth in the Handan Central Hospital from October 2023 to October 2024. The real-time fluorescence quantitative polymerase chain reaction experiment was performed to detect miR-128-3p in tissues and cells. JEG-3 cells were cultured and transfected with anti-miR-128-3p, si-SIRT1, and negative controls, respectively. Tetramethylthiazole blue (MTT) assay was used to detect the viability of JEG-3 cells; transwell experiment was applied to measure the migration and invasion of JEG-3 cells; Western blot was applied to measure expression of cycle-dependent protein kinase inhibitor 1A (P21), matrix metalloproteinase 2 (MMP-2), E-cadherin (E-Cadherin), SIRT1, Cyclin D1(Cyclin D1), HIF-1α protein; dual luciferase assay was used to validate the targeting relationship between miR-128-3p and SIRT1. The experimental results indicate that, the expression level of miR-128-3p in placental tissues of preeclampsia group was higher than that of normal placental tissues (P<0.05). After inhibiting the expression of miR-128-3p, the expression of P21, E-cadherin and HIF-1α decreased, while the cell survival rate, the number of migrating cells and the number of invading cells, and the protein expressions of MMP-2, Cyclin D1 and SIRT1 increased (P<0.05). SIRT1 was the target gene of miR-128-3p, after simultaneously inhibiting the expression of miR-128-3p and SIRT1, the above indicators showed opposite expressions (P<0.05). Overexpression of miR-128-3p may inhibit the proliferation and invasion of gestational trophoblast cells by modulating SIRT1/HIF-1α pathway.
Key words: miR-128-3p; SIRT1; hypoxia inducible factor-1α; preeclampsia; pregnancy trophoblast cell proliferation and invasion
（Acta Laser Biology Sinica, 2025, 34(5): 459-465）