Abstract: This article uses snail enzyme combined with ultrasound to extract Ganoderma lucidum polysaccharides, optimizes its extraction process, and verifies the antioxidant activity of Ganoderma lucidum polysaccharides. Based on single factor experiments, the effects of enzymatic pH, enzymatic temperature, and enzymatic time on the extraction yield of Ganoderma lucidum polysaccharides were investigated, and the optimal process conditions were determined using orthogonal experiments combined with ultrasonic fragmentation method. Three methods, including DPPH, ABTS and FRAP, were used to determine the antioxidant activity of the extracted Ganoderma lucidum polysaccharides. The experimental results showed that the optimal process parameters for extracting Ganoderma lucidum polysaccharides were as follows: enzymatic hydrolysis pH of 5, enzymatic hydrolysis temperature of 50℃, enzymatic hydrolysis time of 2.5 h, ultrasonic crushing for 15 s each time, intermittent for 30 s, and a total of 5 cycles. Under this condition, the extraction yield of Ganoderma lucidum polysaccharides was 1.595%, and the extracted Ganoderma lucidum polysaccharides had good antioxidant activity. This study provides ideas for exploring a high-yield and high antioxidant activity Ganoderma lucidum polysaccharide extraction method.
Key words: Ganoderma lucidum polysaccharide; snail enzyme; ultrasonic crushing method; enzymatic hydrolysis process; antioxidant activity
（Acta Laser Biology Sinica, 2025, 34(5): 417-425）