Abstract: Point-of-care testing (POCT) refers to a category of diagnostic tests that are performed at or near to the site of the patients (also called bedside testing) and is capable of obtaining accurate results in a short time by using portable diagnostic devices, avoiding sending samples to the medical laboratories. It has been extensively explored for diagnosing and monitoring patients’ diseases and health conditions with the assistance of development in biochemistry and microfluidics. Microfluidic paper-based analytical devices (µPADs) have gained dramatic popularity in POCT because of their simplicity, user-friendly, fast and accurate result reading and low cost. Several µPADs have been successfully commercialized and received excellent feedback during the past several decades. This review briefly discusses the main types of µPADs, preparation methods and their detection principles, followed by a few representative examples. The future perspectives of the development in µPADs are also provided.
Key words: point-of-care testing; microfluidic paper-based analytical devices; sensor; personalized medical treatment; portable diagnostic equipment
CLC number: TP212                     Document code: A                      DOI: 10.3969/j.issn.1007-7146.2025.01.001
（Acta Laser Biology Sinica, 2025, 34(1): 001-011）