(1. Agricultural Science Academy of Xiangxi Tujia and Miao Autonomous Prefecture, Xiangxi 416000, China; 2. College of Life and Health Science, Kaili University, Kaili 556011, China; 3. Pingjiang County Agriculture and Rural Bureau Plant Protection and Inspection Station, Yueyang 414500, China）
Abstract: According to the extensive distribution of GYF domain proteins and the diversity of its functions, it is necessary to study GYF domain proteins further and conduct evolutionary analysis. Since AtEXA1 is the most well-studied GYF domain protein in plants, this study mainly focuses on the proteins similar to AtEXA1. Analysis of 8 053 GYF domain proteins in eukaryotes ranging from unicellular lower fungi to higher organisms showed that AtEXA1-like proteins are mainly found in plant species, while GYF proteins from animals have low similarity to AtEXA1. It was also found that AtEXA1-like proteins are present in almost all agricultural crops. This study demonstrates the conservation of all GYF domain proteins, initially deconstructs the evolution of GYF domain proteins, and provides implications for subsequent studies on the functional characteristics of GYF domain proteins.
Key words: GYF domain; conserved domain; protein evolution; protein function
（Acta Laser Biology Sinica, 2024, 33(3): 253-258）