Abstract: To investigate the expression of GSDMB and GSDMD in skin cutaneous melanoma (SKCM) and their correlation with prognosis and immune infiltration based on bioinformatics. The expression and variation of GSDMB and GSDMD in SKCM were analyzed by GEPIA2 database and cBioportal database. The prognostic value of GSDMB and GSDMD on SKCM was analyzed by GEPIA2 database. The GSCA database was used to analyze the correlation between the expression level of GSDMB and GSDMD in SKCM and the activity of tumor-related pathways. The TIMER database was used to analyze the relationship between the expression level of GSDMB and GSDMD in SKCM and the infiltration level of immune cells. The results showed that the expression level of GSDMB in SKCM tissue was significantly lower than that in normal tissue, while the expression level of GSDMD was significantly higher than that in normal tissue. In 363 SKCM samples, the total variation rate of GSDMB and GSDMD was 9.64%, with mutations predominating (4.96%). The high expression level of GSDMB and GSDMD was significantly associated with the higher overall survival rate of SKCM patients. GSDMB may activate apoptosis pathway in SKCM, while GSDMD may activate Hormone ER pathway and apoptosis pathway in SKCM. The expression level of GSDMB and GSDMD was positively correlated with the infiltration level of various immune cells. This study shows that the high expression of GSDMB and GSDMD in SKCM patients is related to the higher infiltration level of immune cells and overall survival rate. GSDMB and GSDMD are expected to become biomarkers related to immune cell infiltration and prognosis of SKCM.
Key words: skin cutaneous melanoma; gasdermin; prognosis; immune infiltration; bioinformatics
（Acta Laser Biology Sinica, 2023, 32(3): 282-288）