Abstract: Based on bioinformatics analysis, a transcription factor FOXM1-derived tumor antigen peptide M1-10 was screened out. Through the experiments of lymphocyte proliferation, interferon gamma release, and cell killing, we found that M1-10 peptide could stimulate the immune memory in vivo and produce better effects when combined with HJURP, MELK, VEGFR1, and VEGFR2-derived four antigens. Tumor pre-immunization experiments were carried out with the mouse models of grafted breast cancer and MMTV-PyMT primary breast cancer. Compared with the control group, M1-10 alone produced significant anti-tumor effects, and M1-10 plus four antigen generated stronger anti-tumor effects. In conclusion, the study developed the tumor antigen peptide M1-10 with a strong immunogenicity, which could result in effective tumor immunity when used alone or combined with other tumor antigen peptides. This conclusion provides further insights into the antitumor mechanism of FOXM1.
Key words: tumor antigen peptide M1-10; transcription factor FOXM1; immune memory; tumor immunity; mouse
（Acta Laser Biology Sinica, 2022, 31(2): 121-128）