Abstract: Chloride channel protein (ClC) is an extremely widely distributed anion channel that exists on the cell membrane of organisms. It plays a very important function in various physiological processes such as salt stress resistance. Using bioinformatics methods, 10 SbClCs genes were identified in the sorghum genome, and the SbClCs gene family was analyzed by genome identification, promoters, conservative motifs, phylogenetic trees and gene expression. The results showed that the SbClCs gene family is mainly distributed on six chromosomes, SBI-01, SBI-03, SBI-04, SBI-06, SBI-07 and SBI-10; SbClCs proteins are all hydrophobins and have good fluidity. The secondary structure is mainly random coils and α-helix; the analysis results of gene protein domains showed that the relatively conservative domains are Voltage gated and CBS, and ClC family members are relatively conservative in protein. After analyzing the cis-acting elements of SbClCs gene promoters, it was found that many light-responsive elements (Sp1, G-box) existed. Except for the SbClCg1 gene, the promoter regions of other genes all have drought-induced-related MYB binding sites (MBS); system phylogenetic tree analysis showed that sorghum ClC is more closely related to corn and rice; gene expression heat map analysis showed that SbClCc3 and SbClCa genes play an important role in salt stress and stress tolerance of sorghum. Selection evolution analysis showed that ClC protein coding genes are also subject to purification selection. This study preliminarily analyzed the structure and expression of 10 chloride ion transporter genes in sorghum, which provided a basis for further research on genetic transformation and molecular breeding with SbClCs.
Key words: sorghum (Sorghum bicolor); ClC; gene expression; phylogeny; molecular breeding
（Acta Laser Biology Sinica, 2022, 31(1): 087-095）