Influence of Low-level Laser Irradiation via Wnt/β-catenin Signal Pathway on RKO Colorectal Cancer Cells

LIU Yufeng, LIU Shaojie, ZHU Weicong, YANG Xiaohong, ZHANG Hanshuo, LI Zhenpeng

Acta Laser Biology Sinica ›› 2020, Vol. 29 ›› Issue (5) : 438-445.

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PDF(4122 KB)
Acta Laser Biology Sinica ›› 2020, Vol. 29 ›› Issue (5) : 438-445.

Influence of Low-level Laser Irradiation via Wnt/β-catenin Signal Pathway on RKO Colorectal Cancer Cells

  • LIU Yufeng, LIU Shaojie, ZHU Weicong, YANG Xiaohong, ZHANG Hanshuo, LI Zhenpeng
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Abstract

Abstract: Normal expression of the Wnt/β-catenin signal pathway plays an important role in regulation of normal cell growth and differentiation, and research on target sites in the Wnt/β-catenin signal pathway thus has become a hotspot on targeted anticancer therapy. Low-level laser irradiation (LLLI) was discussed for its influence on RKO colorectal cancer cells through the Wnt/β-catenin signal pathway. RKO cells were irradiated by the 632.8 nm infrared laser, and RT-PCR method was adopted for screening gene expressions of APC、β-catenin and DVL in the Wnt/β-catenin signal pathway of RKO cells irradiated by a gradient low-level laser. Results of the RT-PCR showed that the low-level laser with an energy density of 15 J/cm2 reduced β-catenin expression of RKO cells, and increased APC and DVL expressions, indicating both an increasing trend as the rising energy density and a statistical discrepancy. Related indicators of RKO cells were tested for their changes by qRT-PCR and Western blot methods, under the low-level laser irradiation of 15 J/cm2. Results of qRT-PCR showed that gene expressions of Wnt5A, LRP5 and TCF-4 indicated different levels of reduction, with a statistical discrepancy. Results of Western blot showed that reduction in protein expression of β-catenin, relative to that of the blank control group, had a statistically significant discrepancy. Both qRT-PCR and Western blot showed that low-level laser irradiation of 15 J/cm2 for short time still had an inhibiting effect on overexpression of Wnt/β-catenin signal pathway of RKO cells. By observation on low-level laser irradiation of 15 J/cm2 through viable and dead-cell kits staining, it can be seen that the dead RKO cells in experimental group were more than that in control group. In conclusion, low-level laser irradiation of 15 J/cm2 can delay proliferation of RKO colorectal cancer cells through inhibition on overexpression of Wnt/β-catenin signal pathway, and is dose-dependent with a time-accumulation effect; it can also activate the apoptosis pathway and therefore incur cell apoptosis. This paper, in view of the above, provided a new thinking for the treatment of colorectal cancer.
Key words: low-level laser therapy; low-level laser irradiation; colorectal cancer cells; RKO cells; Wnt/β-catenin signaling pathway

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LIU Yufeng, LIU Shaojie, ZHU Weicong, YANG Xiaohong, ZHANG Hanshuo, LI Zhenpeng. Influence of Low-level Laser Irradiation via Wnt/β-catenin Signal Pathway on RKO Colorectal Cancer Cells[J]. Acta Laser Biology Sinica. 2020, 29(5): 438-445
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