Abstract: In order to establish African swine fever virus droplet digital PCR detection method with high specificity, high sensitivity and good repeatability, three pairs of primers and probes were synthesized based on two conserved sequences of African swine fever virus nucleic acid, VP72 and K205 genes. In this study, the method was optimized and the linearity, specificity, sensitivity and repeatability were compared. It was found that the detection limits of the three pairs of primers and probes were all less than 6 copies / reaction, and there was a good linear relationship between 10~106 copies / reaction. The coefficients of variation within the quantitative range were all less than 15%. Moreover, synthesized three pairs of primers and probes did not cross-react with the two common viruses of pigs. This method is suitable for the detection of African swine fever virus in pork and its products. It is beneficial to detect African swine fever virus in fresh pork and various pork products at various area such as source, food processing, and food sales, and effectively reduce potential risks.