Olfactory related proteins of insects play an important role in their behaviors and physiological activities such as foraging, mating, defense, spawning, information exchange and habitat selection Fluorescence competitive binding assay is a typical method for detecting the binding properties of insect OBPs and CSPs with environmental odor molecules In this paper, some researches and applications  on fluorescence competitive binding assay used for insects olfactory proteins (OBPs and CSPs) are reviewed It provides  theoretical reference for further exploration on insects olfactory recognition mechanism and formulation strategies on pests control and economic insects protection