Exosomes contain different bimolecular characteristics that reflected components from the released cells, and become an important substance for liquid biopsy. To discriminate different cellderived exosomes, surfaceenhanced Raman spectroscopy (SERS) has been applied for the detection of the cancer cellderived (MCF-7) exosome and normal cellderived(MCF-10A)exosomes. The exosomes were isolated from the supernatant of cell culture medium. The results showed that exosomes had their own unique characteristic Raman spectrum in the region of 800~1 800 cm^-1. Compared with the spectrum from MCF-10Aderived exosomes, the relative Raman peak intensity of the nucleic acid from MCF-7derived exosomes was significantly higher and some the relative Raman peak intensity of lipid decreased or the Raman peak disappeared. The results concluded SERS technology can detect subtle molecular changes in exosomes from different cells with high sensitivity, and distinguish between breast cancer cellderived exosomes and normal cellderived exosomes. SERS technology can provide a fast, labelfree and nondestructive detection approach for the early diagnosis of cancer.