Using five different lines of Brassica napus with similar genetic backgrounds but different seed colors as materials, the changes of zeatin riboside (ZR), auxin (IAA) and abscisic acid (ABA) contents and their regulatory roles during the development of rapeseed were investigated to study the effects of plant hormones on content and component of oil in canola. The results showed that ZR increased  10〖KG-*3〗~20 days after pollination. The content of zeatin riboside (ZR) in yellow seeds accumulated to a higher level than that in black seeds; IAA accumulated in large quantities in seeds, and its content in yellow seeds was significantly higher than that in black seeds 2025 days after pollination; ABA concentration also showed an upward trend, and then gradually accumulate. It declined slightly after and then maintained at a certain level. After 10 days of pollination, the oleic acid content in canola seeds increased gradually. The oleic acid and linoleic acid content in yellow seeds were significantly higher than those in black seeds, and the oleic acid accumulation efficiency was also significantly higher than that in blackseeded ones. The oil content of yellow seeds is significantly higher than that of black seeds at 0.01 level. The results showed that higher concentrations of ZR and IAA in the early and middle stages of seeds development were beneficial to the accumulation of oleic acid, linoleic acid and rape oil.