Softening of fruits and vegetables is a crucial factor limiting their postharvest quality and commercial value, including species and varieties, respiration rate, the production of ethylene, degradation of cell wall, microorganism etc. This paper reviews regulation methods of postharvest fruits and vegetables softening at home and abroad, including physical regulation, chemical regulation, and biological regulation, in order to provide the theoretical foundations for the improvement of postharvest fruits and vegetables quality.