Observation on Body Colour Development in Redwhite Crucian Carp and Expression Analysis of Pigment Genes in Different Color Crucian Carp

Acta Laser Biology Sinica ›› 2019, Vol. 28 ›› Issue (4) : 343-352.

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PDF(5081 KB)
Acta Laser Biology Sinica ›› 2019, Vol. 28 ›› Issue (4) : 343-352.

Observation on Body Colour Development in Redwhite Crucian Carp and Expression Analysis of Pigment Genes in Different Color Crucian Carp

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The early body color formation of redwhite crucian carp was observed. Melanocytes firstly appear before fertilization, then xanthophores happen and later iridophore appear. And then, the body colour of redwhite crucian carp changes from dark grey to redorange after about 3 months old. the fulllength cDNAs for Slc7a11 and Pnp4a were isolated. Our results revealed that the fulllength Pnp4a cDNA contains 1 535 bp, with an open reading frame of 783 nucleotides, which encodes a deduced protein of 291 amino acids, coding for a deduced sequence alignment showed that crucian carp Pnp4a shares high levels of identity with those from Danio rerio (95.1% ). The fulllength Slc7a11 cDNA contains 1 782 bp, with an open reading frame of 1488 nucleotides, which encodes a deduced protein of 496 amino acids. The amino acid sequence alignment demonstrated that the red crucian carp Slc7a11 protein shared a sequence identity of 93.7% with Danio rerio, Oryzias latipes.The expression of Mitfa,Tyr,Slc7a11 and Pnp4a in three kinds of different color crucian carp embryos and adult tissues were detected by RTPCR. The results showed that the redwhite crucian carp has the same melanin decay process as the red crucian carp.Amino acid sequence alignment showed that  Pnp4a and Slc7a11of red crucian carp share high identity with those from Cyprinus carpio.The adult skin tissue of the redwhite crucian carp was detected the expression of Mitfa and Tyr,so did the adult skin tissue of the red crucian carp.Pnp4a was expressed in different body color development stages and different tissues of different crucian carp.Slc7a11 was expressed in different body color development stages of different crucian carp. However,it was not detected in different adult skin tissues of different crucian carp.

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Observation on Body Colour Development in Redwhite Crucian Carp and Expression Analysis of Pigment Genes in Different Color Crucian Carp[J]. Acta Laser Biology Sinica. 2019, 28(4): 343-352
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