gpr98 is associated with a variety of diseases, and it is highly conserved in human and zebrafish. Constructing the gpr98 gene mutant strain of zebrafish can provide an excellent animal model and research basis for explaining the function of the gpr98 gene. In this paper, two target sites with a distance of 42 bp in exon 2 of zebrafish gpr98 gene were selected for sgRNA recognition. SgRNA was synthesized in vitro and coinjected with Cas9 mRNA into the embryo of zebrafish at 1cell stage. A few partial embryos were randomly selected for DNA genotyping when they are in 72hour stage. The results showed that in addition to the wildtype DNA bands, some embryos have a band smaller than the wildtype DNA bands. The F0 positive individuals were mated with wildtype zebrafish, and the offspring of the F0 generation positive individuals were tested for genotyping, and a stable genetic mutation strain with a deletion of 48 bp was obtained. The construction of the mutants model lays an important foundation for the study of the role of gpr98 gene in the development of cardiovascular, skeletal and other tissues and organs as well as the occurrence of related diseases.