The application value of twodimensional speckletracking echocardiography (2DSTE) in evaluating the left atrial function in patients with hyperthyroidism was explored. During the period from June 2015 to June 2018, a total of 200 subjects were selected for this study. Out of 200 subjects, 100 were the patients with hyperthyroidism in our hospital, and 100 were apparently healthy individuals from the physical examination center (ascertained as controls). Three left atrial index were measured by 2DSTE, including the left atrial volume (LAVmax), minimum volume (LAVmin), left atrial presystolic volume (LAVp), function (LAPEF) and left atrial active ejection fraction (LAAEF), strain ［global strain rate (sRs), early diastolic strain rate (sRe) and late diastolic strain rate (sRa)］, which demonstrated the value of 2DSTE in evaluating the left atrial function in patients with hyperthyroidism. Compared with the healthy group, the LAPEF, LAAEF were significantly decresed in the hyperthyroidism group, while the LAVmax, LAVmin and LAVp significantly incresed. The differences were statistically significant (P<0.05). The sRs of the hyperthyroidism group was obviously lower than that in the healthy group, while the sRe and sRa in the hyperthyroidism group were significantly higher than those of the healthy group. The differences were statistically significant (P<0.05). The Pearson correlation analysis showed that the sRs were positively correlated with LAPEF and LAAEF, the sRe, sRa were negatively correlated with LAPEF and LAAEF (P<0.05). In this study, the results demonstrated that 2DSTE is an excellent technology in evaluating the left atrial function in patients with hyperthyroidism, and sRs, sRe, sRa can be used as the important indexes in evaluating the left atrial function of patients, which is worth for further clinical promotion.