The oil content of rapeseed is important for improving oil yield And the traditional method for new oilseed rape materials is complex and timeconsuming In order to find a new method for rapid diagnosis of oilseed rape breeding materials, 3 groups of 9 different oil content Brassica napus lines were uesed as raw materials, meanwhile, hyperspectral reflectance and chlorophyll were tested in different stage and the relationship between them was analyzed The results showed that the hyper spectral reflectance of rape displays two peaks and three valleys in the visible band Meanwhile, in rapeseed seedling stage, the higher oil content rapeseed hed a lower hyperspectral reflectanceThe chlorophyll a was the main reason which led to the difference of total chlorophyll content There was a negative correlation at 490 nm and 670 nm between the spectral reflectance and chlorophyll content, the correlation coefficient was -0898 and -0783 respectively There was a significant positive correlation between chlorophyll and oil content, which reached to 0962There was a negative correlation between reflectivity and oil content at 490 nm, 560 nm and 670 nm, and the correlation coefficient was -0949, -0962 and -0937 respectively At last, the oil content of rapeseed can be predicted by Hyper spectral method in seedling stage and provide reference for rape breeding with high oil content These results can be used to choose high oil content rapeseed materials in early stages, which can enhance the breeding process and promote new materities breeding While, the samples in this study is small, and samples will be enlarged  to improve the  prediction accuracy of oil content by Hyper spectral method