This study was to observe the repair effect of recombinant human collagen(rhc)on laser induced damage of mice skin and to explore preliminarily its mechanism.Dorsal skin of mice was irradiated with 458~514 nm laser, rhc dissolved in physiological saline solution(8 mg/mL)was used to treat dorsal skin wounds of mice,  once per day for 14 days. The collagen fibers in the wound dermis were observed by using the backwards second harmonic generation(SHG)signals which were collected by two photon microscopy(1,4,7,14 d)after the administration. The pathological changes of the wound were observed by routine HE staining. The effects of rhc on proliferation of epidermic cells and fibroblasts were studied in vitro, and cell viability was calculated.The results indicated  that compared with the control group, rhc significantly accelerated wound healing and shortened wound healing time in mice. SHG imaging showed that rhc could promote the production of collagen in wound, and in vitro experiments rhc showed  markedly proliferationpromoting activity for human skin fibroblasts and keratinocytes. It can be concluded that rhc(8 mg/mL)has a repairing effect on laserdamaged mouse skin, which may be achieved by stimulating the proliferation of epidermal keratinocytes and dermal fibroblasts and promoting collagen deposition.