ASB11 is involved in the development of embryonic neural progenitor cells, regenerative myogenesis and ubiquitination, but its detailed mechanism of action has not been known. In order to further analyze the mechanism of zebrafish Asb11 gene, the antiASB11 specific polyclonal antibody by immunizing mice with DNA was prepared. The eukaryotic expression vector pCAGGSP7/ASB11 was constructed by using cDNA of zebrafish Asb11. BALB/c mice of 68 weeks old were immunized with pCAGGSP7/ASB11 to prepare antiserum against ASB11. The results showed that the pCAGGSP7/ASB11 recombinant plasmid was constructed with a rather good immunogenicity. The antibody valence of antiserum obtained from the immunized mice was 1〖DK〗∶400. Western blotting and indirect immunofluorescence showed that the antiserum prepared by immunizing mice with DNA could specifically recognize the ASB11 protein. This research established  afoundation to reveal the mechanism of ASB11 protein.