Objective： To evaluate the clinical efficacy and the quality of life of patients with alopecia areata treated by the CO₂ fractional lasersassisted drug transdermal delivery technique combined with topical 5% minoxidil tincture. Methods： 46 cases of alopecia areata were randomly divided into the observation group and the control group, with 23 cases in each group. The observation group was treated with CO₂ fractional laser treatment, 1 times every 2 weeks, a total of 6 times, at the same time with topical 5% Minoxidil Tincture, 2 times a day. In the control group, only 5% Minoxidil Tincture was used externally and the usage was the same as the observation group. After 12 weeks of treatment, the clinical efficacy, adverse reaction and dermatology life quality index (DLQI) of the two groups were observed and compared. Results： The total effective rate was 91.30% in the observation group and 65.22% in the control group, the difference was statistically significant(P<0.05). Before treatment, there was no significant difference in DLQI scores between the two groups (t=-0.912, P=0.367). After treatment, the DLQI scores were significantly lower than those before treatment (t=-10.42, P=0.000; t=-8.614, P=0.000), DLQI scores decreased significantly in the observation group compared to the control group (t=-2.6.36, P=0.012). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusions： Fractional CO₂ laserassisted drug transdermal delivery technique combined with 5% Minoxidil Tincture in treatment of alopecia areata has significant clinical efficacy, mild adverse reactions, The method is simple, safe, worthy of clinical application.