Macrophage polarization plays an important role in bone repair. Once being implanted in the body, bone repair materials will trigger response of macrophage, making it switch to M1/M2 phenotype, thus affect the osteogenic effect of the material. In this work, Strontiumsubstituted micro/nano bioactive glasses （SrMNBG）with 0, 5%, 10% and 15% molar percent of strontium element were prepared, and the effect of SrMNBG on macrophage polarization was studied. Subsequently, the effects of SrMNBG with different strontium incorporation ratio on the osteogenic differentiation of murine bone marrow mesenchymal stem cells (mMSCs) were evaluated. Finally, the in vivo assessment was conducted to verify the polarization of macrophage and osteogenesis of SrMNBG through in situ bone defect repair experiment. The in vitro results showed that compared with 0%SrMNBG，SrMNBG with 5%, 10% and 15% molar percent of strontium element could further promote macrophage polarization toward M2, and 10%SrMNBG displayed the best effect. Moreover, the secretion of macrophages modulated by 10%SrMNBG was more beneficial to osteogenesis. The in vivo results showed that there were more M2 macrophages around 10%SrMNBG after implantation in the body, and better bone repair effect was found in the 10%SrMNBG group, which were consistent with the in vitro results. Therefore, SrMNBG is expected to be a promising immunoregulatory material for bone repair.