Nuclear receptor subfamily 6 group member1 (Nr6a1) is an active ligand transcription factor member of the nuclear receptor superfamily, also known as germ cell nuclear factor (GCNF) or retinoic acid receptorrelated testisassociated receptor (RTR). As a transcription factor, Nr6a1 plays a role of transcriptional inhibition by binding to the DR0 element (TCAAGGTCA) in the target gene promoter region. Nr6a1 is mainly expressed in mature germ cells, differentiated embryonic stem cells and germ cell tumors such〖JP1〗 as teratoma, which suggested that the expression profile of Nr6a1 is spatiotemporal specificity and tissue specificity. To date, a number of upstream or downstream of Nr6a1 regulatory molecules including miR181a, let7, Oct4, PPARδ have been identified. Through the interactions with these genes, Nr6a1 plays an important regulating role in biological processes of development, cell differentiation and metabolism.