Transcription factor GATA1 plays a key role in the differentiation of normal erythroid cells, GATA1 deletion caused the apoptosis of primary erythrocytes, Overexpression of GATA1 prevents late differentiation of erythrocytes. This experiment is aimed at studying how to effect the development of blood vascular. In order to prepare GATAI polyclonal antibody, the study amplificated gata1 coding region of zebrafish by reverse transcription PCR, constructed pCAGGSP7/gata1 eukaryotic expression plasmid and adopted DNA Immunization technique to immunize mice. Detection of target protein expression by Western blot and immunofluorescence 〖JP2〗technology. Experimental results showed that the eukaryotic recombinant expression plasmid pCAGGSP7/gata1 was successfully constructed. The recombinant plasmid has good immunogenicity, Antiserum obtained after immunization of mice, Antibody titer up to 1〖DK1〗∶500, Western blot and immunofluorescence confirmed that antiserum can specifically recognize GATA1 protein, A firm foundation for the further study of gata1 function.