In the previous research, it was found that Plateletderived growth factorBB (PDGFBB) was closely related to coronary heart disease. Here, 60 urine samples from patients with coronary heart disease including 20 patients with PCI (percutaneous coronary intervention) and 40 patients without PCI and 18 urine samples from healthy human were investigated based on surfaceenhanced Raman spectroscopy (SERS). It revealed that the peak at 1 509 cm1 attributing to PDGFBB could be detected in the urine samples from the patients with PCI and cannot be detected in the urine samples from healthy human and most of patients without PCI. Compared with the clinical data, it was shown that the SERS spectra of urine samples were in good agreement with the results of coronary angiograph technology in determining whether cardiovascular congestion was above 70%. The measurement of human urine based on SERS could be developed as a noninvasive and prospective diagnostic tool for coronary heart disease, and it provided viable clinic information for judging the suspected cases of coronary heart disease and deciding whether performing PCI.