In order to investigate biological function of soybean Lea5 gene, one of LEA gene family, the overexpression vector of pBI121Lea5 was constructed. Through leaf discs transformation by Agrobacterium (LB4404:pBI121Lea5), four transgenic plants of TL06, TL09, TL17 and TL32 were selected and identified. Analysis by RTPCR shows that soybean Lea5 gene was expressed in T2 generation plants of the four transgenic lines in different level. Treated with drought and salinity, T2 generation plants of TL09 and TL32 displayed more resistance to drought and more tolerance to salinity. This indicates that overexpression of soybean Lea5 gene confers resistance to drought and tolerance to salinity in plant.