To investigate the expression pattern of Gsk3β gene in different developmental stages and adult tissues of red crucian carp, as well as its molecular response under cadmium stress. 〖WTHZ〗Methods:〖WTBZ〗The full length of Gsk3β cDNA was obtained by RACE cloning technique, which Gsk3β mRNA and protein was analyzed by semiquantitative RTPCR and Western Blot.The expression of Gsk3β was analyzed by specific concentration of cadmium. 〖WTHZ〗Results: 〖WTBZ〗The total length of Gsk3β gene was 2 195 bp,the 5′untranslated region (UTR) was 692 bp, and the 3′ untranslated region was 237 bp. Encoding 421 amino acids containing protein and protein molecular weight was 46 846.40, its isoelectric point was 9.05,and has the closest relationship with zebrafish and medaka.Gsk3β gene was expressed in different embryonic developmental stages and adult tissues.The expression of Gsk3β was most abundant in the brain tissue, and the expression of Gsk3β in the muscle and testis was higher than in the liver and kidney, ovaries and skin tissues. At different developmental stages, Gsk3β was not expressed in the twocell stage, and then expressed at different stages of followup. The expression of Gsk3β was the strongest in the last stage, followed by the duration of muscle, In the eye pigment period and somatic stage protein expression is weak. The expression of Gsk3β related genes were detected in the liver of red crucian carp after cadmium treatment, and the result showed that expression level of Gsk3β was in downregulation. 〖WTHZ〗Conclusion: 〖WTBZ〗Gsk3β gene plays an important role in different embryonic developmental stages of red crucian carp and participates in cadmium stress response, but its specific molecular mechanism remains to be further studied.