In this research, genome sequencing data of Photorhabdus luminescens TT01 strains exhibited six secret genes related to the expression of the toxin complexes (Tc), including tcaZCB (plu05140515), tccAB3 (plu08050806), tccAB1 (plu41654169), tcd (plu09600971), tccC4 (plu0976) and tccC7 (plu4488), respectively. Firstly, four larger genes in TT01 chromosome named tcaZCB (6 804 bp), tccAB3 (7 419 bp), tccAB1 (10 954 bp) and tcd (51 406 bp) were rapidly fished out and constructed into vectors p15AcmtcaZCB, p15AcmtccAB3, p15Acm tccAB1, p15Acmtcd via direct cloning method. Then another two genes, tccC4 (2 891 bp) and tccC7 (2 871 bp) were gained by PCR amplification technique and formed recombinant vectors pMD18tcc4 and pMD18tcc7 by ligation with Tvector. Finally, using two different biotechnology methods, six episomal Tc genes in TT01 chromosome were successfully cloned. which would provide a positive foundation for the further study of different Tc toxins.