In order to provide reference for the researchers and technologies on how to use the Laser scanning confocal microscope efficiently, taking a set of Laser confocal laser scanning microscope (the model is TCSSP8) which was produced by Leica corporation in Germany as an example, a lot of suggestions and introdutctions about function, principle, structure, use of sofeware, instrumental management, experimental technology and  analysis of common problems in use were disserted in this paper