CRISPR/Cas9 is a versatile gene engineering technology that has been received extensive attention  in gene editing, regulation of gene expression and live cell DNA/RNA imaging as well. However, the CRISPR/Cas9 system is derived from prokaryotes, which requires delivery vehicles for application in other organisms. Combined with a variety of applications of CRISPR/Cas9 technology, this paper reviews its delivery vehicles to providing a reference to the work of relevant fields.