Objective:〖WTBZ〗To investigate the value of T lymphocyte ELISA dot experiment (TSPOT), tuberculin skin test (TST) and tuberculosis antibody in the diagnosis of.patients with pulmonary tuberculosis in silicosis. 〖WTHZ〗Methods:〖WTBZ〗Fortyone cases of silicosis TB infection, ninety 〖HJ2.1mm〗cases of non silicosis tuberculosis, forty cases of healthy medical students as control group were collected from August 7, 2015 to May 20, 2016, all the above samples were detected by the above three immunological methods, and the resuts of different methods were analyzed. 〖WTHZ〗Result:〖WTBZ〗1) the positive rates of TSPOT in silicosis, non silicosis tuberculosis and healthy people were 73.17%, 93.33% and 15% respectively, there were significant differences between the two groups in the three methods:The positive rates of TST in silicosis, non silicosistuberculosis and healthy people were 58.54%, 88.89% and 32.5% respectively, compared with non silicosis TB patients, the differences were statistically significant. There were no significant differences between healthy people and silicosis patients, Compared with non silicosis patients, the differences between healthy people and non silicosis patients were statistically significant. The positive rates of TB antibody in silicosis, non silicosis, tuberculosis and healthy people were 36.58%, 42.22% and 52.5%, respectively. There were no statistically significant differences between the two groups in the three methods. 2) the sensitivity of the three detection methods in silicosis patients was 73.17%, 58.54% and 36.58%, respectively, and the differences were statistically significant:The sensitivity of TSPOT and TST was higher than that of TB antibody, the differences were statistically significant, the specificity was 85%, 67.5% and 47.5%, respectively, the differences were statistically significant, The specificity of TSPOT was higher than that of tuberculosis antibody method, the difference was statistically significant; the positive predictive value were 83.33%, 64.86%, 41.67%, and negative predictive value were 75.56%, 61.36%, 64.44%, the differences between positive and negative predictive value of three methods, were statistically significant. 3) the positive rate of TSPOT in I, II, III phase tuberculosilicosis detection was 52.38%, 94.12%, 100%, there were significant difference between the positive rate of I and II phase or III phase, there were no significant difference between II and III positive rate. 〖WTHZ〗Conclusion:〖WTBZ〗TSPOT has high sensitivity and specificity, and is an effective methodin the diagnosis and clinical staging of silicosis.