GYF domains are small, conservative, versatile adaptor domains that recognize prolinerich sequences (PRS). GYF domain proteins play important roles in protein protein interaction, translational initiation, mRNA splicing, surveillance and translation suppression, ubiquitin ligation, signal transduction and immune protease regulation, et al. The study progress of GYF domain protein on aspects of domain characters, interaction with target proteins as well as its function were reviewed in the paper.