摘 要：为探讨长链非编码RNA（lncRNA）BCAN-AS1在舌鳞状细胞癌中的表达以及BCAN-AS1/miR-1224-5p/Wnt轴调控舌鳞状细胞癌细胞增殖和侵袭的机制，本研究用cBioPortal数据库分析舌鳞状细胞癌组织中BCAN-AS1的表达，利用qRT-PCR检测BCAN-AS1在人正常口腔角质细胞（HOK）和舌鳞状细胞癌细胞（Tca8113、HNl3、SCC-9、TSCCA、SCC-15）中的表达。体外培养SCC-9细胞，并将其分为Con组（转染阴性质粒）和si-BCAN-AS1组（转染si-BCAN-AS1质粒）。利用克隆形成试验和Transwell试验分别检测各组SCC-9细胞的增殖和侵袭能力。利用双荧光素酶报告试验验证BCAN-AS1和miR-1224-5p的互补结合。利用cBioPortal数据库分析舌鳞状细胞癌组织中BCAN-AS1和miR-1224-5p表达的相关性。利用qRT-PCR检测miR-1224-5p在各组SCC-9细胞中的表达。利用Western blot法检测SCC-9细胞中Wnt/β-catenin通路蛋白的表达。与癌旁组织比较，BCAN-AS1在舌鳞状细胞癌组织中表达较高（P<0.01）。与HOK细胞相比，BCAN-AS1在Tca8113、HNl3、SCC-9、TSCCA、SCC-15细胞中表达均较高（P<0.01）。si-BCAN-AS1组细胞增殖能力和侵袭能力均低于Con组（均P<0.01）。BCAN-AS1可靶向结合miR-1224-5p（P<0.01）。舌鳞状细胞癌组织中BCAN-AS1和miR-1224-5p的表达呈现明显的负相关（P<0.01）。干扰BCAN-AS1表达后，SCC-9细胞中miR-1224-5p表达升高（P<0.01），Wnt/β-catenin信号通路蛋白β-catenin、Cyclin D1、с-mус、MMP-7表达均下降（均P<0.01）。舌鳞状细胞癌中BCAN-AS1高表达，下调BCAN-AS1可通过miR-1224-5p/Wnt/β-catenin轴调控舌鳞状细胞癌SCC-9细胞的增殖和侵袭。本研究表明，BCAN-AS1可能成为舌鳞状细胞癌靶向治疗的分子靶点。

关键词：BCAN-AS1；舌鳞状细胞癌；口腔角质细胞；miR-1224-5p；Wnt/β-catenin通路
中图分类号：R739.86                          文献标志码：A                  DOI：10.3969/j.issn.1007-7146.2025.05.011

Abstract: To investigate the expression of long noncoding RNA (lncRNA) BCAN-AS1 in tongue squamous cell carcinoma (TSCC) and the mechanism by which BCAN-AS1/miR-1224-5p/Wnt axis regulates the proliferation and invasion of TSCC cells, cBioPortal database was used to analyze BCAN-AS1 expression in TSCC tissues. qRT-PCR was used to detect the expression of BCAN-AS1 in normal human oral keratinocytes (HOK) and TSCC cells (Tca8113, HNl3, SCC-9, TSCCA, SCC-15). SCC-9 cells were cultured in vitro and divided into Con group (transfected with negative plasmid) and si-BCAN-AS1 group (transfected with si-BCAN-AS1 plasmid) according to the treatment method. The proliferation and invasion abilities of SCC-9 cells in each group were detected by clone formation assay and Transwell assay. Dual luciferase reporter assay verified the complementary combination between BCAN-AS1 and miR-1224-5p. The cBioPortal database was used to analyze the correlation between BCAN-AS1 and miR-1224-5p expressions in TSCC tissues. qRT-PCR was used to detect miR-1224-5p expression in SCC-9 cells in each group. Western blot detected Wnt/β-catenin pathway proteins expression in SCC-9 cells in each group. Compared with adjacent tissues, BCAN-AS1 was highly expressed in TSCC tissues (P<0.01). Compared with HOK cells, BCAN-AS1 was highly expressed in Tca8113, HNl3, SCC-9, TSCCA, and SCC-15 cells (P<0.01). The cell proliferation and invasion abilities of the si-BCAN-AS1 group were lower than those of the Con group (all P<0.01). BCAN-AS1 could target and bind to miR-1224-5p (P<0.01). miR-1224-5p expression in SCC-9 cells increased (P<0.01), and the expressions of β-catenin, Cyclin D1, с-mус, and MMP-7 proteins decreased (all P<0.01). BCAN-AS1 is highly expressed in TSCC, and downregulation of BCAN-AS1 can regulate the proliferation and invasion of TSCC SCC-9 cells through the miR-1224-5p/Wnt/β-catenin axis. BCAN-AS1 may become a molecular target for targeted therapy of TSCC.
Key words: BCAN-AS1; tongue squamous cell carcinoma; oral keratinocytes; miR-1224-5p; Wnt/β-catenin pathway
（Acta Laser Biology Sinica, 2025, 34(5): 473-480）