摘 要：为保障经颅激光照射的温度安全性，观察808 nm激光在不同光照时间和功率下经颅照射活体小鼠头部时对头皮和下颌温度的影响及安全参数，并检测加装水冷装置是否能有效降低照射温度。将808 nm准直激光对准小鼠前囟照射，用红外热成像仪测量头皮温度，用热电偶测量下颌温度。将C57BL/6J小鼠分为3组：A组（自然冷却组）、B组（主动冷却组）、C组（阴性对照组）。A组又根据照射功率的不同分为A1、A2、A3 3个小组。记录各组照射剂量为～140 J时小鼠头皮和下颌的温度变化，结果发现：A组的头皮温度升高4.2℃，B组升高2.4℃，B组较A组降低1.8℃；A组的下颌温度升高3.0℃，B组升高2.4℃。研究表明，808 nm激光经颅照射小鼠头部时，随着平均功率的增加，局部照射的温度升高速度加快。激光照射在小鼠头皮上的平均功率为～300 mW、照射剂量为～140 J对小鼠是安全的。当需要更高的照射功率时，可使用局部冷却的方法有效降低局部温度。研究结果可为小鼠经颅照射治疗设定参数范围提供试验依据。
关键词：808 nm激光；照射剂量；小鼠；经颅照射；光生物调节
中图分类号：Q631                        文献标志码：A                    DOI：10.3969/j.issn.1007-7146.2025.04.004

Abstract: To evaluate the thermal safety of transcranial photobiomodulation, this study investigates the effects of 808 nm laser irradiation on scalp and mandibular temperatures, as well as safety parameters, when applied to the heads of live mice under varying illumination durations and power levels. Additionally, to evaluate whether a water-cooling device can effectively reduce irradiation-induced temperature increases. The 808 nm laser was directed at the fontanelle of mice. Scalp temperatures were measured using an infrared thermal imager, and jaw temperatures were recorded using a thermocouple. C57BL/6J mice were divided into three groups: Group A (natural cooling), Group B (active cooling), and Group C (negative control). Group A was further subdivided into A1, A2, and A3 based on different power levels of irradiation. Changes in scalp and mandibular temperatures were recorded at an irradiation dose of～140 J. Scalp temperature increased by 4.2℃ in Group A and by 2.4℃ in Group B, with Group B showing a temperature reduction of 1.8℃ compared to the corresponding power level in Group A . Jaw temperature measurements indicated a rise of 3.0℃ in Group A and 2.4℃ in Group B. As the average power of the 808 nm laser increased, local temperatures rose significantly. An average irradiation power of approximately 300 mW and a dose of ～140 J were found to be safe for mouse transcranial irradiation. For higher power levels, active cooling effectively mitigated local temperature increases. The results of this study can provide experimental basis for setting the parameter range of transcranial irradiation in mice.

Key words: 808 nm laser; exposure dose; mice; transcranial irradiation; photobiomodulation
（Acta Laser Biology Sinica, 2025, 34(4): 312-318）