摘　要：为探讨长链非编码RNA（lncRNA）HHIP-AS1对舌鳞状细胞癌细胞增殖和迁移的影响及其分子机制，本文通过cBioPortal数据库分析HHIP-AS1在舌鳞状细胞癌组织中的表达，并利用实时荧光定量聚合酶链反应（qPCR）检测HHIP-AS1在舌鳞状细胞癌细胞系中的表达。将HHIP-AS1质粒和对照质粒转染至CAL-27细胞，分别记为HHIP-AS1组和NC组。利用qPCR检测HHIP-AS1在各组CAL-27细胞中的表达。利用细胞计数试剂盒-8（CCK-8）法和划痕愈合试验分别检测上调HHIP-AS1对CAL-27细胞增殖和迁移的影响。利用蛋白质印迹（Western blot）法检测上调HHIP-AS1对CAL-27细胞增殖表型蛋白质和迁移表型蛋白质表达的影响。利用双荧光素酶报告试验验证HHIP-AS1和miR-19a-3p的靶向关系，qPCR检测上调HHIP-AS1对miR-19a-3p表达的影响。结果显示：HHIP-AS1在舌鳞状细胞癌细胞系中表达均低于人正常口腔角质HOK细胞（均P<0.05）；HHIP-AS1组CAL-27细胞中HHIP-AS1表达显著高于NC组（P<0.01）。本研究发现，上调HHIP-AS1能显著抑制CAL-27细胞增殖能力（P<0.05）和迁移能力（P<0.01），上调HHIP-AS1会抑制CAL-27细胞增殖表型蛋白质和迁移表型蛋白质表达。本研究进一步发现，HHIP-AS1靶向结合miR-19a-3p（P<0.01），并且上调HHIP-AS1会抑制miR-19a-3p表达（P<0.01）。综上所述，HHIP-AS1在舌鳞状细胞癌中低表达，上调HHIP-AS1通过降低miR-19a-3p表达抑制舌鳞状细胞癌细胞的增殖和迁移。本研究可能为舌鳞状细胞癌的靶向治疗研究提供新的靶点。
关键词：HHIP-AS1；舌鳞状细胞癌；miR-19a-3p；癌细胞增殖；癌细胞迁移
中图分类号：R739.86　　　          文献标志码：A                   DOI：10.3969/j.issn.1007-7146.2025.03.011

Abstract: To investigate the effects of long noncoding RNA (lncRNA) HHIP-AS1 on the proliferation and migration of tongue squamous cell carcinoma cells and its molecular mechanism, the expression of HHIP-AS1 in tongue squamous cell carcinoma tissues was analyzed by cBioPortal database. The expression of HHIP-AS1 in tongue squamous cell carcinoma cells was detected by quantitative real-time polymerase chain reaction (qPCR). HHIP-AS1 plasmid and control plasmid were transfected into CAL-27 cells, which were recorded as HHIP-AS1 group and NC group, respectively. qPCR was used to detect the expression of HHIP-AS1 in CAL-27 cells in each group. Cell counting kit-8 (CCK-8) method and scratch healing assay were used to detect the effects of up-regulating HHIP-AS1 on the proliferation and migration of CAL-27 cells, respectively. Western blot was used to detect the effects of up-regulating HHIP-AS1 on the expression of proliferation phenotype proteins and migration phenotype proteins in CAL-27 cells. Dual luciferase reporter assay was used to verify the targeting relationship between HHIP-AS1 and miR-19a-3p. qPCR was used to detect the effect of upregulating HHIP-AS1 on the expression of miR-19a-3p. The results showed that the expression of HHIP-AS1 in tongue squamous cell carcinoma cell lines was lower than that in human normal oral keratinocytes (HOK cells) (all P<0.05). The expression of HHIP-AS1 in CAL-27 cells of the HHIP-AS1 group was significantly higher than that in the NC group (P<0.01). This study found that upregulating HHIP-AS1 could significantly inhibit the proliferation ability (P<0.05) and migration ability (P<0.01) of CAL-27 cells. Upregulating HHIP-AS1 would inhibit the expression of proteins related to the proliferative phenotype and migratory phenotype of CAL-27 cells. This study further found that HHIP-AS1 could bind to miR-19a-3p in a targeted manner (P<0.01), and upregulating HHIP-AS1 would inhibit the expression of miR-19a-3p (P<0.01). In conclusion, HHIP-AS1 is lowly expressed in tongue squamous cell carcinoma. Upregulating HHIP-AS1 inhibits the proliferation and migration of tongue squamous cell carcinoma cells by reducing the expression of miR-19a-3p. This study may provide a new target for the research on targeted therapy of tongue squamous cell carcinoma.
Key words: HHIP-AS1; tongue squamous cell carcinoma; miR-19a-3p; cancer cell proliferation; cancer cell migration
（Acta Laser Biology Sinica, 2025, 34(3): 282-288）