摘　要：光谱流式细胞仪作为近年来发展迅速的一款检测仪器，目前主要的应用场景是对传统流式细胞仪的补充。本研究通过三个光谱流式细胞仪在荧光材料检测中的试验，展示了光谱流式细胞仪在生物材料领域中的应用前景。研究表明，光谱流式细胞仪不仅可以探索已知荧光染料的非主要波段荧光，还可以通过解析荧光材料与细胞凋亡检测试剂盒中荧光染料及荧光探针的发射光谱重叠区域，实现多组分信号的精准分离。此外，它还能够检测荧光纳米材料在细胞内的光谱位移。概括来说，光谱流式细胞仪不仅能够记录荧光材料进入细胞内的光谱特征，分析细胞内荧光基团的光谱情况，还能对与荧光检测通道重复的荧光信号进行光谱拆分，从而提供客观的荧光结果。这些功能为荧光材料的研究开辟了新的分析思路，并为光谱流式细胞仪在生物材料科学中的广泛应用提供了理论依据和实践参考。
关键词：光谱流式细胞仪；生物材料；荧光材料；荧光光谱移动；光谱拆分
中图分类号：Q631　　　　　　文献标志码：A　　　　　　DOI：10.3969/j.issn.1007-7146.2025.03.004

Abstract: As a detection instrument developed rapidly in recent years, the spectral flow cytometer primarily complemented traditional flow cytometry. This article demonstrated the performance of three spectral flow cytometers in the detection of fluorescent materials, demonstrating their potential applications in the field of biological materials. Studies showed that the spectral flow cytometer could not only explore fluorescence from non-primary bands of known fluorescent dyes, but also decompose overlapping spectra between fluorescent materials, and reagent kit detection channels, resulting in more accurate analytical results. Additionally, it could detect the spectral shifts of fluorescent nanomaterials within cells. In all, the spectral flow cytometer could record the spectral characteristics of fluorescent materials entering cells, analyze the spectral behavior of fluorophores within cells, and spectrally resolve fluorescence signals that overlapped with detection channels, thus providing objective fluorescence data. These capabilities opened up new paths for the research of fluorescent materials, providing theoretical foundations as well as practical references for the widespread use of spectral flow cytometry in biomaterials science.
Key words: spectral flow cytometry; biological materials; fluorescent materials; fluorescence spectrum shift; spectral separation
（Acta Laser Biology Sinica, 2025, 34(3): 221-228）