（秦皇岛市北戴河医院骨二科，秦皇岛 066100）
摘　要：为探究冬凌草甲素对人软骨肉瘤细胞迁移和侵袭能力的影响，体外培养人软骨肉瘤SW-1353细胞，将试验分别设为对照组（不做干预），1.25、2.50、5.00 μmol/L冬凌草甲素组（1.25、2.50、5.00 μmol/L冬凌草甲素）和阳性药物组（6 μmol/L阿霉素），检测人软骨肉瘤SW-1353细胞的活力、迁移率、侵袭能力、上皮-间质转化（EMT）蛋白表达量。结果显示：与对照组比较，5.00 μmol/L冬凌草甲素组和阳性药物组人软骨肉瘤SW-1353细胞活力降低（P<0.05）；1.25、2.50、5.00 μmol/L冬凌草甲素组和阳性药物组迁移率及侵袭能力降低，且浓度越高变化越显著（P<0.05）。冬凌草甲素能够通过升高E-钙黏蛋白（E-cadherin）的表达、降低波形蛋白（vimentin）和N-钙黏蛋白（N-cadherin）的表达量进而抑制EMT进程，且浓度越高变化越显著（P<0.05）。冬凌草甲素可显著抑制人软骨肉瘤SW-1353细胞的迁移和侵袭，或是通过介导EMT实现的，这为人软骨肉瘤细胞的生物学研究提供了理论基础。
关键词：冬凌草甲素；软骨肉瘤；SW-1353；迁移；侵袭
中图分类号：R739.96                                 文献标志码：ADOI：10.3969/j.issn.1007-7146.2024.03.012

(Department of Orthopaedics, Beidaihe Hospital of Qinhuangdao, Qinhuangdao 066100, China)
Abstract: To investigate the effects of oridonin on migration and invasion of human chondrosarcoma cells. In vitro experiments, human chondrosarcoma SW-1353 cells were divided into control group being without intervention, 1.25, 2.50, 5.00 μmol/L oridonin groups (1.25, 2.50, 5.00 μmol/L oridonin) and positive drug group (6 μmol/L adriamycin). Human chondrosarcoma SW-1353 cells were tested for viability, mobility, invasion ability and epithelial-mesenchymal transition (EMT) expression. The results show that: compared with the control group, SW-1353 cell viability in the 5.00 μmol/L oridonin group and positive drug group significantly decreased (P<0.05). 1.25, 2.50, 5.00 μmol/L oridonin groups and positive drug obviously inhibted cell migration and invasion in a concentration-dependent manner (P<0.05). The expression of E-cadherin decreased the expression of vimentin and N-cadherin and inhibited EMT process by increasing the expression of E-cadherin protein, and the higher the concentration, the more significant the change was (P<0.05). Lamonidin can significantly inhibit the migration and invasion of human chondrosarcoma SW-1353 cells, or through mediating EMT, which provides a theoretical basis for the biological study of human chondrosarcoma cells.
Key words: oridonin; chondrosarcoma; SW-1353; migration; invasion
（Acta Laser Biology Sinica, 2024, 33(3): 284-288）