摘　要：为了解湖南西洞庭湖国家级自然保护区两栖动物的资源状况及受威胁因素，于2016—2018年选取保护区内典型生境，设置10条样线开展实地调查，记录样线内环境因子、两栖动物种类和数量及威胁因子。结果显示：共记录两栖动物14种，隶属1目5科8属，占湖南省已记录68种两栖动物的20.59%；区系组成多为东洋界种类，其中，东洋界华中区、华南区共有种9种，占64.29%；东洋界华中区、华南区、西南区共有种4种，占28.57%；被列为国家II级重点保护野生动物的有虎纹蛙（Hoplobatrachus chinensis）。经过计算分析，记录到的两栖动物Shannon-Wiener指数和Simpson指数分别为1.445 4和0.649 1，Pielou均匀度为0.149 4。相关性分析表明，不同月份两栖动物的物种丰富度及相对多度与环境因子均无显著相关性。湖南西洞庭湖国家级自然保护区两栖动物资源较丰富，但分布不均匀，多种人为干扰因素对该地区两栖动物多样性具有潜在影响。
关键词：西洞庭湖国家级自然保护区；两栖动物；物种多样性；人为干扰；调查
中图分类号：Q958.1　　　　　      文献标志码：ADOI：10.3969/j.issn.1007-7146.2021.03.010

Abstract: Amphibians are important indicators of ecological environment monitoring. In order to understand the amphibian resource status and threatened factors in the National Nature Reserve of West Dongting Lake, Hunan province, from 2016 to 2018, typical habitats in the reserve were selected and 10 sample lines were set to carry out field survey, and environmental factors, amphibian species, number and threat factors within the sample line were recorded. The results showed that: 14 species of amphibians were recorded, belonging to 1 order, 5 families and 8 genera, accounting for 20.59% of the 68 species of amphibians recorded in Hunan province. Most of the floras are Oriental species, among which there are 9 species in central China and south China, accounting for 64.29%. There were 4 species in the southwest area of south China and central China in the eastern boundary, accounting for 28.57%. Hoplobatrachus chinensis classified as National II Key Wildlife Conservation. After calculation and analysis, the recorded amphibian Shannon-Wiener index and Simpson index were 1.445 4 and 0.649 1 respectively, and the Pielou evenness was 0.149 4. Correlation analysis showed that the species richness and relative abundance of amphibians in different months were not significantly correlated with environmental factors. The amphibian resources in West Dongting Lake National Nature Reserve in Hunan province are abundant, but they are not evenly distributed and a variety of human disturbance factors have potentials impacts on the diversity of amphibians in the region.
Key words: West Dongting Lake National Nature Reserve; amphibians; species diversity; human disturbances; investigation
（Acta Laser Biology Sinica, 2021, 30(3): 276-283）