摘　要： MYB-CC基因家族是由MYB-DNA结合域和CC（coiled-coil）结构域组成的，其中CC结构域能通过折叠参与蛋白质之间的相互作用，是一个二聚体结构域。这类MYB-CC转录因子是植物特有的，除磷饥饿响应蛋白1（PHR1）外，拟南芥中另外14个蛋白也同样具有这两个结构域。部分MYB-CC基因已经被证明可调控植物中磷酸盐吸收和控制磷饥饿反应。为了探索谷子MYB-CC基因家族的特征和功能，本文通过生物信息学分析来解析谷子MYB-CC基因家族的特征，分析其表达模式及谷子与水稻、玉米基因组中的MYB-CC基因家族的共线性、选择压力。结果表明：通过基因在谷子染色体上的定位情况，共筛选到谷子MYB-CC基因家族基因18个，分布在7条染色体上；基序分析发现存在1个相对保守的基序motif 1；顺式作用元件分析发现，参与防御和胁迫响应相关的MYB-CC基因有6个；基因表达模式分析结果显示，SiMYB-CC6在根和茎秆中特异性表达。该研究结果可为谷子MYB-CC基因家族的进一步研究提供一定的参考依据。
关键词：谷子；MYB-CC基因家族；生物信息学；基因表达；低磷胁迫
中图分类号：S515　　　　　　文献标志码：ADOI：10.3969/j.issn.1007-7146.2021.03.008

Abstract: MYB-CC gene family is transcription factor containing MYB-DNA binding domain and CC (coiled-coil) domain. The CC domain is a dimer domain that can participate in the interaction with proteins through structure folding. This is unique in plants that contain this domain of MYB-CC transcription factor. In addition to phosphate starvation response1 (PHR1), another 14 proteins in Arabidopsis thaliana also have these two domains. Some MYB-CC genes have shown to regulate phosphate uptake and control phosphate starvation in plants. In order to explore the characteristics and functions of MYB-CC gene family in foxtail millet, the characteristics and expression patterns of MYB-CC gene family in foxtail millet were analyzed by bioinformatics analysis. Finally, the collinearity and selection pressure of MYB-CC gene family in foxtail millet, rice and maize genomes were analyzed. The results showed that 18 genes of MYB-CC family were found on 7 chromosomes; a relatively conservative motif 1 was found in the motif analysis; cis-elements analysis showed that there were 6 MYB-CC genes involved in defense and stress response. The results of gene expression analysis showed that SiMYB-CC6 was specifically expressed in roots and stems. The results can provide reference for the further study of MYB-CC genes family in foxtail millet.
Key words: foxtail millet; MYB-CC gene family; bioinformatics; gene expression; low phosphorus stress
（Acta Laser Biology Sinica, 2021, 30(3): 259-269）