摘　要：为了研究皮肤组织深层和浅层血供对植皮手术术后恢复的影响，本研究构建了大鼠深层缺血皮肤对照模型和多因素大鼠浅层皮肤对照模型，并利用激光散斑衬比成像技术对皮肤血运状态进行监测。在模型中，通过控制不同区域的供血情况，以相对血流灌注指数为指标，测量并量化皮肤移植区域的血流状态，同时通过组织血流灌注比来分析术后皮肤的恢复趋势。结果表明：在大鼠深层缺血皮肤对照模型中，股动脉结扎组的组织血流灌注比低于正常组；在多因素大鼠浅层皮肤对照模型中，进行缺血和烧烙操作的试验组血流灌注比低于对照组。本研究通过构建两种动物模型，证明了激光散斑衬比成像技术能够在植皮术后实时监测血流情况，皮肤深层组织和浅层组织的血供会直接影响术后区域的恢复效果。
关键词：激光散斑衬比成像；皮肤移植；血供；血流灌注；大鼠
中图分类号：R331                             文献标志码：A                   DOI：10.3969/j.issn.1007-7146.2025.01.004

Abstract: To investigate the influence of deep and superficial blood supply of skin tissue on post-grafting recovery, rat models of deep ischemic skin and multifactorial rat models of superficial skin were constructed in study. Laser speckle contrast imaging technique was utilized to monitor the skin perfusion status. In the models, the relative blood perfusion index was measured and quantified in the skin grafting area by controlling the blood supply in different regions, while the tissue perfusion ratio was analyzed to assess postoperative skin recovery trends. The results indicated that in the rat model of deep ischemic skin, the tissue perfusion ratio of the femoral artery ligature group was lower than that of the normal group. In the multifactorial rat model of superficial skin, the experimental groups subjected to ischemia and cauterization showed lower blood perfusion ratios compared to the control group. By establishing two animal models in study, it was demonstrated that the laser speckle contrast imaging technique can provide real-time monitoring of blood flow after grafting surgery. The blood supply to both deep and superficial skin tissues directly affects the postoperative recovery of the area.
Key words: laser speckle contrast imaging; skin graft; blood supply; blood perfusion; rats
（Acta Laser Biology Sinica, 2025, 34(1): 026-035）