摘　要：表皮生长因子（EGF）作为一种细胞保护肽，在肠道生长和健康中起着重要的作用。本研究主要探讨EGF对断奶仔猪肠道抗氧化能力、炎症反应和免疫状态的影响。选取42头21日龄的断奶仔猪随机分配到3个处理组中，这些处理组均由相同的基础日粮组成，分别含有0（对照）、200或400 µg/kg EGF。每个处理14个重复，并且在试验的第7天和第14天对每个处理的7头仔猪进行采样。在整个试验期间，膳食补充200 µg/kg EGF增加了超氧化物歧化酶（SOD）的活性。这种补充也降低了断奶后第7天丙二醛（MDA）的含量，但增加了血清免疫球蛋白A（IgA）的含量。在整个试验期间，补充400 µg/kg EGF降低了肿瘤坏死因子-α（TNF-α）的浓度，并倾向于提高分泌型免疫球蛋白A（SIgA）的水平。此外，饲喂EGF日粮的仔猪核因子-κB（NF-κB）p65的磷酸化水平相对更高。综上，EGF可以提高断奶仔猪的肠道抗氧化能力，减少炎症反应，并提高免疫状态，这表明EGF对仔猪肠道健康有积极作用。

关键词：表皮生长因子；抗氧化能力；免疫力；炎症；断奶仔猪

中图分类号：S828.9                           文献标志码：ADOI：10.3969/j.issn.1007-7146.2024.01.007

（Acta Laser Biology Sinica, 2024, 33(1): 057-064）

Abstract: Epidermal growth factor (EGF) is a cytoprotective peptide that plays a crucial role in gut growth and health. The study mainly explored the effects of EGF on the intestinal antioxidant capacity, inflammatory response, immune status in weaning piglets. Forty-two 21-day-old weaned piglets were randomly assigned to three treatments consisting of a same basic diet containing 0 (control), 200, or 400 µg/kg EGF, respectively. There were 14 replicates per treatment, and 7 piglets per treatment were sampled on days 7 and 14 of the experiment. Dietary supplementation of 200 µg/kg EGF increased the activity of superoxide dismutase (SOD) during the entire experimental period. This supplementation decreased malondialdehyde (MDA) content whereas it increased serum immunoglobulin A (IgA) content on day 7 post-weaning. Animals receiving the diet supplemented with 400 µg/kg EGF decreased concentration of tumor necrosis factor-α (TNF-α) and tended to increase the level of secretory immunoglobulin A (SIgA) in the overall experimental period. In addition, the phosphorylation level of nuclear factor-κB (NF-κB) p65 was higher for piglets fed EGF diet. In summary, EGF can enhance intestinal antioxidant capacity, decrease inflammatory response, and increase immune status in weaned piglets, suggesting that EGF has a positive role in piglet gut health.

Key words: epidermal growth factor (EGF); antioxidant capacity; immunity; inflammation; weaning piglets

CLC number: S828.9                     Document code: ADOI:10.3969/j.issn.1007-7146.2024.01.007