摘　要：小眼畸形相关转录因子（MITF）与黑色素细胞的形成关系密切。casper突变体斑马鱼是一种nacre与roy orbison杂交选育得到的双基因突变斑马鱼品系（mitfaw2/w2; roya9/a9）。通过对casper斑马鱼的鱼鳍和鳞片以及早期体色发育过程的观察，证实了casper斑马鱼的臀鳍、背鳍与背部鳞片均无黑色素细胞，并且在早期体色发育的过程中，也未有黑色素细胞生成。构建mitfa过表达重组质粒，并将其显微注射至casper斑马鱼受精卵中，在其发育过程中观察到黑色素细胞的产生。结果进一步证实mitfa在鱼类体色形成尤其是在黑色素细胞发生方面发挥着重要作用。同时，mitfa过表达casper斑马鱼的制备，也为深入开展鱼类体色分子调控机制研究提供了材料平台。
关键词：斑马鱼；mitfa；黑色素；casper突变体；基因过表达
中国分类号：Q78     　　　 　　文献标志码：ADOI：10.3969/j.issn.1007-7146.2021.05.007

Abstract: Microphthalmia-related transcription factors (MITF) are closely related to the formation of melanocytes (melanophores). The casper mutant zebrafish is a double-gene mutant zebrafish strain (mitfaw2/w2; roya9/a9) bred by crossing nacre and roy orbison. The observation of fin, scale and early body color development of mitfa-mutated casper zebrafish in this paper confirmed that there were no melanocytes in anal fin, dorsal fin and dorsal scale of casper zebrafish, and no melanocytes were generated during the early body color development. The recombinant plasmid of mitfa overexpression was constructed and microinjected into the fertilized eggs of casper zebrafish. Melanocytes were observed in the head, back and abdomen of the injected group. The results further confirmed that mitfa plays an important role in the formation of fish body color, especially in the formation of melanocytes. At the same time, the preparation of mitfa over-expressing casper zebrafish also provides a material platform for in-depth research on the molecular regulation of fish body color.
Key words: zebrafish; mitfa; melanophores; casper mutant; gene over-expression
（Acta Laser Biology Sinica, 2021, 30(5): 429-434）