摘 要：本文用波长1 341 nm的Nd:YAP激光辐照亚心形扁藻（Platymonas subcordiformis），培养一段时间后，利用Lambda 950紫外-可见-近红外分光光度计测量藻液的吸收光谱，并通过透射电子显微镜（TEM）观察扁藻的超微结构，分析激光辐照对亚心形扁藻色素吸光度及其细胞内含物（如淀粉粒和脂滴等）的影响。结果表明，激光辐照扁藻没有改变色素的最大吸收波长，但是却改变了它的吸光度，同时使细胞内淀粉粒数量和脂滴都有不同程度的变化，这为后续进行激光选育优质藻株提供了理论和实践依据。
关键词：亚心形扁藻；吸收光谱；吸光度；淀粉粒；脂滴
中图分类号：Q631      文献标志码：A      DOI：10.3969/j.issn.1007-7146.2026.03.009

Abstract: This study irradiated the marine alga Platymonas subcordiformis with a 1 341 nm Nd:YAP laser. After a period of cultivation, the absorption spectrum of the algal suspension was measured using a Lambda 950 UV-Vis-NIR spectrophotometer, and the ultrastructural changes of the algae were observed by transmission electron microscopy (TEM). The aim was to analyze the influence of laser irradiation on the absorbance of algal pigments and intracellular components, such as starch grains and lipid droplets. The experimental results indicated that while laser irradiation did not alter the maximum absorption wavelengths of the pigments, it significantly affected their absorbance. Concurrently, variations were observed in the number of starch grains and the quantity of lipid droplets within the cells. This provides a theoretical and practical basis for the subsequent selection of high-quality algal strains through laser breeding.
Keywords: Platymonas subcordiformis; absorption spectrum; absorbance; starch grains; lipid droplets
（Acta Laser Biology Sinica, 2026, 35(3): 270-274）