（1. 华南师范大学光电科学与工程学院，生物光子学研究院，激光生命科学教育部重点实验室，广州 510631；2. 华南师范大学光电科学与工程学院，生物光子学研究院，广东省激光生命科学重点实验室，广州 510631)
摘 要：皮肤老化表现为真皮层变薄、弹性纤维退化、胶原流失及表皮更新减缓。光生物调控疗法（PBMT），特别是红光波段照射，在皮肤抗衰领域已经展现出良好应用前景，但其分子机制尚不完全明确。本研究建立自然老化小鼠模型（18～20月龄），采用红光照射（630～635 nm，能量密度8 J/cm2，功率密度6.67 mW/cm2，20 min/次，连续28 d），考察了其对皮肤衰老的改善效应及其机制。结果显示，红光显著增加老化小鼠真皮层厚度，促进真皮层I型胶原蛋白沉积。机制研究表明：红光通过激活AKT信号通路促进成纤维细胞胶原合成；同时，AKT信号激活可刺激角质形成细胞增殖（Ki67表达增加）与分化（细胞角蛋白10表达增加），且该效应可被AKT抑制剂API-2阻断。上述结果表明，红光通过AKT信号通路实现真皮-表皮协同重塑，为光生物调控疗法在皮肤老化防治中的应用提供了理论依据。
关键词：光生物调节作用；红光；皮肤老化；AKT信号通路；胶原蛋白；角质形成细胞
中图分类号：R454.2                     文献标志码：A                    DOI：10.3969/j.issn.1007-7146.2026.02.009

Abstract: Skin aging is characterized by dermal atrophy, degeneration of elastic fibers, collagen depletion, and attenuated epidermal turnover. Photobiomodulation therapy (PBMT), particularly red light irradiation, has demonstrated promising anti-aging potential; however, its underlying molecular mechanisms remain incompletely elucidated. In this study, we established a natural aging mouse model (18～20 months) to investigate the rejuvenating effects and mechanisms of red light treatment with parameter of 630～635 nm, fluence 8 J/cm2, power density 6.67 mW/cm2, 20 min per session for 28 consecutive days. Our results demonstrated that red light significantly increased dermal thickness and enhanced type I collagen deposition in aged mice. Mechanistic investigations revealed that red light promoted collagen synthesis in fibroblasts via activation of the AKT signaling pathway; concurrently, AKT activation stimulated keratinocyte proliferation with elevated Ki67 expression, and differentiation with increased cytokeratin 10 expression, effects that were abrogated by the AKT inhibitor API-2. These findings collectively indicate that red light mediates coordinated dermal-epidermal remodeling through AKT signaling, providing a theoretical foundation for the application of photobiomodulation therapy in the prevention and treatment of skin aging.
Key words: photobiomodulation; red light; skin aging; AKT signaling pathway; collagen; keratinocyte
（Acta Laser Biology Sinica, 2026, 35(2): 179-184）