摘 要：基于Janus激酶（JAK）/信号转导与转录激活因子（STAT）通路，探究穴位贴敷联合加味八珍汤对骨髓抑制小鼠骨髓造血功能的影响。将BALB/c小鼠随机分为对照组、模型组、穴位贴敷组、加味八珍汤组、联合用药组，每组10只。尾静脉采血检测外周血血红蛋白、白细胞、血小板水平，酶联免疫吸附测定（ELISA）检测血清促红细胞生成素（EPO）、促血小板生成素（TPO）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的水平，各组小鼠进行骨髓有核细胞计数。各组小鼠的骨髓有核细胞进行粒系造血祖细胞（CFU-GM）、红系造血祖细胞（CFU-E）和巨核系祖细胞（CFU-Meg）的细胞集落数检测。试剂盒检测血清中超氧化物歧化酶（SOD）、丙二醛（MDA）水平，蛋白质印记（Western blot）检测小鼠骨髓有核细胞中磷酸化JAK1（p-JAK1）、JAK1、p-JAK2、JAK2、p-STAT3、STAT3蛋白的表达。结果发现：相对于对照组，模型组小鼠血红蛋白、白细胞、血小板、EPO、TPO、GM-CSF、SOD、骨髓有核细胞数量及造血祖细胞CFU-GM、CFU-E、CFU-Meg的细胞集落数降低（P<0.05），MDA、p-JAK1/JAK1、p-JAK2/JAK2、p-STAT3/STAT3升高（P<0.05）；相对于模型组，穴位贴敷组、加味八珍汤组、联合用药组小鼠血红蛋白、白细胞、血小板、EPO、TPO、GM-CSF、SOD、骨髓有核细胞数量及造血祖细胞CFU-GM、CFU-E、CFU-Meg的细胞集落数升高（P<0.05），MDA、p-JAK1/JAK1、p-JAK2/JAK2、p-STAT3/STAT3降低（P<0.05）；穴位贴敷与加味八珍汤能够协同增效（P<0.05）。研究表明，穴位贴敷联合加味八珍汤能够协同增效，通过减轻机体氧化应激，改善了骨髓抑制小鼠的骨髓造血功能，其机制可能与抑制JAK/STAT信号通路相关。

关键词：穴位贴敷；加味八珍汤；骨髓抑制；Janus激酶/信号转导与转录激活因子信号通路；氧化应激
中图分类号：R285.5                 文献标志码：A      DOI：10.3969/j.issn.1007-7146.2026.01.010

Abstract: Acupoint application combined with Modified Bazhen Decoction mediated the effect of Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway on bone marrow hematopoietic function in mice with bone marrow inhibition. The BALB/c mice were randomly divided into control group, model group, acupoint application group, Modified Bazhen Decoction group, and combined drug group, with 10 mice in each group. Tail vein blood was collected to measure the levels of hemoglobin, white blood cells, and platelets in peripheral blood. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum erythropoietin (EPO), thrombopoietin (TPO) and granulo-monocyte colony-stimulating factor (GM-CSF). Bone marrow nucleated cell counts were performed in each group of rats. For the myeloid nucleated cells of each group, colony forming unit-granulocyte and macrophage (CFU-GM), colony forming unit-erythrocyte (CFU-E) and colony forming unit-megakaryocyte, erythrocyte and granulocyte (CFU-Meg) were detected. The kit was used to detect the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum. The expression of p-JAK1, JAK1, p-JAK2, JAK2, p-STAT3, STAT3 proteins in mouse nucleated bone marrow cells were detected by Western blot. The results showed that compared with the control group, the number of hemoglobin, leukocytes, platelets, EPO, TPO, GM-CSF, SOD, bone marrow nucleated cells and the number of cell colonies of hematopoietic progenitor cells CFU-GM, CFU-E and CFU-Meg in the model group decreased (P<0.05), and the MDA, p-JAK1/JAK1, p-JAK2/JAK2, p-STAT3/STAT3 increased (P<0.05). Compared with the model group, the number of hemoglobin, leukocytes, platelets, EPO, TPO, GM-CSF, SOD, bone marrow nucleated cells and the number of cell colonies of hematopoietic progenitor cells CFU-GM, CFU-E and CFU-Meg increased in the acupuncture point application group, Modified Bazhen Decoction group and combined drug group (P<0.05), and the MDA, p-JAK1/JAK1, p-JAK2/JAK2, p-STAT3/STAT3 decreased (P<0.05). Acupoint application and Modified Bazhen Decoction could synergize (P<0.05). It indicated that acupoint application combined with Modified Bazhen Decoction synergistically improves the bone marrow hematopoietic function of mice with bone marrow suppression by reducing oxidative stress, and its mechanism may be related to the inhibition of JAK/STAT signaling pathway.
Key words: acupoint application; Modified Bazhen Decoction; bone marrow inhibition; Janus kinase/signal transducer and activator of transcription signaling pathway; oxidative stress
（Acta Laser Biology Sinica, 2026, 35(1): 088-096）