摘 要：本文主要探讨环状RNA（circRNA）circKRT75在舌鳞状细胞癌（TSCC）组织中的表达及沉默其表达对TSCC细胞增殖和侵袭的影响。采用cBioPortal数据库分析circKRT75在TSCC组织中的表达，以及circKRT75表达水平与TSCC患者生存期的相关性。实时荧光定量聚合酶链反应（qPCR）检测circKRT75在TSCC细胞系H357、Tca8113、TSCCA、HNl3、SCC-9中的表达。通过脂质体转染技术分别将si-circKRT75质粒和对照质粒转染进H357细胞，定义为si-circKRT75组和si-NC组。利用噻唑蓝（MTT）法和Transwell法分别检测低表达circKRT75对H357细胞增殖和侵袭的影响。利用Western blot法检测低表达circKRT75对H357细胞增殖表型蛋白细胞周期蛋白依赖性激酶2（CDK2）、细胞周期蛋白E（Cyclin E）、基质金属蛋白酶-2（MMP-2）、α-平滑肌肌动蛋白（α-SMA）表达的影响。利用双荧光素酶报告试验验证circKRT75和miR-128-3p的靶向关系。采用cBioPortal数据库分析circKRT75与miR-128-3p在TSCC组织中表达的相关性。利用qPCR检测低表达circKRT75对miR-128-3p表达的影响。结果显示，circKRT75在TSCC组织中表达高于正常口腔黏膜组织（P<0.01）。circKRT75表达水平与TSCC患者生存期呈负相关（P<0.01）。与人正常口腔角质HOK细胞相比，TSCC细胞系H357、Tca8113、TSCCA、HNl3、SCC-9中circKRT75均呈现高表达（均P<0.05）。与si-NC组相比，低表达circKRT75可抑制H357细胞增殖能力（P<0.05）和侵袭能力（P<0.01）。低表达circKRT75可下调H357细胞中Cyclin E、CDK2、MMP-2、α-SMA蛋白的表达（P<0.01）。circKRT75与miR-128-3p存在靶向关系（P<0.01）。TSCC组织中circKRT75表达与miR-128-3p表达呈负相关（P<0.01）。与si-NC组相比，低表达circKRT75可上调miR-128-3p表达（P<0.01）。综上所述，TSCC组织和细胞系中circKRT75表达水平高，低表达circKRT75通过调控miR-128-3p表达抑制TSCC细胞的增殖和侵袭，circKRT75可能成为TSCC的分子治疗靶点。

关键词：环状RNA；舌鳞状细胞癌；微小RNA；细胞增殖；细胞侵袭
中图分类号：R739.86                         文献标志码：A                      DOI：10.3969/j.issn.1007-7146.2026.01.009

Abstract: To investigate the expression of circular RNA (circRNA) circKRT75 in tongue squamous cell carcinoma (TSCC) tissues and the effects of silencing its expression on the proliferation and invasion of TSCC cells. The cBioPortal database was used to analyze the expression of circKRT75 in TSCC tissues and the correlation between circKRT75 expression and survival in TSCC patients. Quantitative real-time polymerase chain reaction (qPCR) was used to detect circKRT75 expression in TSCC cell lines H357, Tca8113, TSCCA, HN13, and SCC-9. si-circKRT75 and control plasmids were transfected into H357 cells using lipofectamine, defining them as the si-circKRT75 and si-NC groups, respectively. The effects of low-expression circKRT75 on the proliferation and invasion of H357 cells were assessed by MTT and Transwell assays, respectively. Western blot analysis was used to examine the effects of low-expression circKRT75 on the expression of Cyclin E and Cyclin-dependent kinase 2 (CDK2), matrix metalloproteinase-2 (MMP-2) and Alpha-smooth muscle actin (α-SMA) in H357 cells. Dual-luciferase reporter assays were used to validate the targeting relationship between circKRT75 and miR-128-3p. The cBioPortal database was used to analyze the correlation between the expression of circKRT75 and miR-128-3p in TSCC tissues. qPCR was used to detect the effect of low-expression circKRT75 on miR-128-3p expression. circKRT75 expression was higher in TSCC tissues than in normal oral mucosa tissues (P<0.01). circKRT75 expression was negatively correlated with TSCC patient survival (P<0.01). Compared with normal human oral keratinocytes (HOK) cells, circKRT75 expression was higher in TSCC cell lines H357, Tca8113, TSCCA, HN13, and SCC-9 (all P<0.05). Compared with the si-NC group, low circKRT75 expression inhibited the proliferation (P<0.05) and invasion (P<0.01) of H357 cells. Low circKRT75 expression downregulated the expression of Cyclin E, CDK2, MMP-2, and α-SMA proteins in H357 cells (all P<0.01). circKRT75 had a targeting relationship with miR-128-3p (P<0.01). The expression of circKRT75 in TSCC tissues was negatively correlated with the expression of miR-128-3p (P<0.01). Compared with the si-NC group, low expression of circKRT75 upregulated miR-128-3p expression (P<0.01). circKRT75 is highly expressed in TSCC tissues and cell lines, and low expression of circKRT75 inhibits the proliferation and invasion of TSCC cells by regulating miR-128-3p expression. circKRT75 may become a molecular therapeutic target for TSCC.
Key words: circular RNA; tongue squamous cell carcinoma; microRNA; cell proliferation; cell migration
（Acta Laser Biology Sinica, 2026, 35(1): 081-087）