摘 要：为探究白茅素联合甲硝唑对创伤性口腔溃疡的治疗效果及潜在作用机制，选取36只SD大鼠并随机分组，造模成功后用不同药物进行为期7 d的治疗，观察并记录各组大鼠的表征；通过苏木精-伊红染色观察口腔黏膜的病理变化；采用ELISA法检测大鼠血浆中IL-1和IL-6的表达水平。结果显示：造模成功后，各试验组大鼠出现拱背蜷曲、少动萎靡和食欲下降等现象；药物干预后，以上表征得到改善，联合用药高剂量组大鼠的体重最接近空白对照组。观察发现，联合用药高剂量组大鼠口腔溃疡的创面愈合情况最好。苏木精-伊红染色结果显示，模型组大鼠的上皮结构呈病理性改变，用药后，联合用药高剂量组恢复最好。ELISA结果显示，相比白茅素组、甲硝唑组，联合用药高、低剂量组IL-1、IL-6的表达水平降低（P<0.001）。本研究表明，白茅素联合甲硝唑可通过抑制炎症因子IL-1、IL-6的表达水平，对创伤性口腔溃疡具有极好的治疗作用，且效果较单一用药更好。这为口腔溃疡的临床治疗提供了更好的策略。
关键词：创伤性口腔溃疡；白茅素；甲硝唑液；联合用药；炎性因子
中图分类号：R780.2      文献标志码：A              DOI：10.3969/j.issn.1007-7146.2025.06.009

Abstract: To investigate the therapeutic efficacy and underlying mechanisms of cylindrin combined with metronidazole for traumatic oral ulcers, we randomly assigned 36 SD rats to different groups. After successful modeling, the rats received respective drug treatments for 7 days. General conditions of each group were observed and recorded. Pathological changes in oral mucosa were examined via hematoxylin-eosin staining, while plasma levels of IL-1 and IL-6 were detected using ELISA. The results showed that the rats in each group exhibited symptoms after successful modeling, such as hunching and curling, reduced behaviors and lethargy, as well as decreased appetite. After drug intervention, these symptoms improved, for one example, the weight of the high-dose group of combined medication is the closest to the blank control group. It was observed that the oral ulcer wounds in the high-dose group of combined medication showed the best effect by treatment. The result of hematoxylin-eosin staining revealed pathological changes in the epithelial structure of the modeling group, while the high-dose group of combined medication showed the best recovery after treatment. Compared to the cylindrin group and the metronidazole group, the results of ELISA indicated that the expression levels of IL-1 and IL-6 were significantly reduced in both the high and low-dose groups of combined medication (P<0.001). This study demonstrated that the mixed solution of cylindrin and metronidazole suppresses the expression of inflammatory factors IL-1 and IL-6, which contributes a more excellent therapeutic influence than single-agent on traumatic oral ulcers. It is expected to provide a better strategy for the clinical treatment of oral ulcers.
Key words: traumatic oral ulcer; cylindrin; metronidazole solution; combination therapy; inflammatory factor
（Acta Laser Biology Sinica, 2025, 34(6): 551-560）