摘 要：为评估马齿苋提取液和强脉冲光及两者联合对豚鼠激素依赖性皮炎（HDD）的疗效差异，并探讨其作用机制，本研究选取30只豚鼠，随机分为5组，每组6只。其中1组作为空白组，其余4组使用0.05%丙酸氯倍他索软膏建立HDD模型。模型建立后，其中1组作为模型组，其余3组为治疗组，治疗组分别接受马齿苋提取液外用、590 nm强脉冲光治疗以及两者联合治疗3种治疗方式。配合直接观察皮损变化和苏木精-伊红染色观察皮损组织病理形态学改变，利用酶联免疫吸附试验（ELISA）法检测皮损组织中肿瘤坏死因子-α（TNF-α）、白细胞介素-6 （IL-6）、半胱氨酸天冬氨酸酶-14（Caspase-14）、兜甲蛋白（LOR）的表达水平。ELISA结果显示，模型组豚鼠皮损组织中IL-6、TNF-α的表达水平升高，Caspase-14、LOR的表达水平降低（P<0.05），而3个治疗组均出现IL-6、TNF-α的表达水平降低、Caspase-14、LOR的表达水平升高的情况，并且较其他两个治疗组，联合组IL-6、TNF-α的表达水平降低和Caspase-14、LOR的表达水平升高的情况更明显（P<0.05）。研究表明，马齿苋提取液可通过抗炎及修复皮肤屏障发挥改善豚鼠HDD的作用，与强脉冲光联合使用可协同增效。本研究旨在探讨马齿苋提取液与强脉冲光在豚鼠HDD模型中的协同疗效及其潜在的作用机制，为临床治疗提供参考依据。
关键词：激素依赖性皮炎；马齿苋；强脉冲光；豚鼠；协同疗效机制
中图分类号：R751.05                文献标志码：A                  DOI：10.3969/j.issn.1007-7146.2025.06.006

Abstract: To evaluate the efficacy differences of Portulaca oleracea extract, intense pulsed light, and their combination in treating hormone-dependent dermatitis (HDD) in guinea pigs, and to explore their mechanisms of action, this study selected 30 guinea pigs and randomly divided them into 5 groups (6 per group). One group served as the control, while the remaining four groups developed an HDD model using 0.05% clobetasol propionate ointment. After the model was established, one group was designated as the model group, and the other three groups received three treatments: topical application of Portulaca oleracea extract, 590 nm intense pulsed light therapy and the combination of the two. Changes in skin lesions were directly observed, and histopathological alterations were assessed via haematoxylin-eosin staining. Enzyme-linked immunosorbent assay (ELISA) was employed to measure tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), cysteinyl aspartate specific proteinase-14 (Caspase-14) and loricrin (LOR) expression levels in the lesion tissues. The ELISA results revealed elevated IL-6 and TNF-α expression alongside reduced Caspase-14 and LOR expression in the lesion tissues of guinea pigs in the model group (P<0.05). In addition, IL-6 and TNF-α levels were reduced and Caspase-14 and LOR levels were increased in the three treatment groups, and the effect was more significant in the combination group (P<0.05). The study indicates that Portulaca oleracea extract improves guinea pig HDD through anti-inflammatory effects and skin barrier repair, with synergistic enhancement when combined with intense pulsed light. This study aimed to investigate the synergistic therapeutic effects of Portulaca oleracea extract and intense pulsed light in a guinea pig HDD model and elucidate its potential mechanisms of action, thereby providing a reference for clinical treatment.
Key words: hormone-dependent dermatitis; Portulaca oleracea; intense pulsed light; guinea pig; collaborative therapeutic mechanism
（Acta Laser Biology Sinica, 2025, 34(6): 523-529）