摘　要：为探究银杏内酯B对人肺腺癌（A549）细胞增殖和凋亡的影响及IκB激酶/核因子κB（IKK/NF-κB）信号通路的调控作用，体外培养A549细胞，将其分为对照组（相同体积二甲基亚砜溶剂）和不同浓度（4、8、16、32 μmol/L）银杏内酯B组，干预24 h，细胞计数试剂盒-8（CCK-8）检测细胞活力筛选最适浓度的银杏内酯B用于后续试验。随后将A549细胞分为对照组、银杏内酯B组（16 μmol/L银杏内酯B）、阳性药物组（0.4 mg/L阿霉素）、抑制剂组（16 μmol/L银杏内酯B+10 μmol/L IKK/NF-κB通路抑制剂BAY11-7082）和激活剂组（16 μmol/L银杏内酯B+1 μmol/L IKK/NF-κB通路激活剂Prostratin），干预24 h。5-乙炔基-2′脱氧尿嘧啶核苷（EdU）测定细胞增殖能力；Hoechst 33258染色试剂盒测定细胞凋亡能力；蛋白免疫印迹法检测细胞周期蛋白D1（CyclinD1）、半胱氨酸蛋白酶-3（Caspase-3）及IKK/NF-κB通路相关蛋白表达水平。根据CCK-8试验结果，选择16 μmol/L银杏内酯B用于后续试验。与对照组比较，银杏内酯B组和阳性药物组A549细胞增殖率、CyclinD1、p-NF-κB p65/NF-κB p65和p-IκBα/IκBα比值显著降低（P<0.05），凋亡率和Caspase-3蛋白表达水平显著升高（P<0.05）；与银杏内酯B组比较，抑制剂组细胞增殖率、CyclinD1、p-NF-κB p65/NF-κB p65和p-IκBα/IκBα比值显著降低（P<0.05），凋亡率和Caspase-3蛋白表达水平显著升高（P<0.05），激活剂组细胞增殖率、CyclinD1、p-NF-κB p65/NF-κB p65和p-IκBα/IκBα比值显著升高（P<0.05），凋亡率和Caspase-3蛋白表达水平显著降低（P<0.05）。银杏内酯B能通过调控IKK/NF-κB通路信号转导抑制A549细胞的增殖，并诱导其凋亡。
关键词：肺腺癌；银杏内酯B；IκB激酶/核因子κB信号通路；细胞增殖；细胞凋亡
中图分类号：R730    　　　         文献标志码：A　　　    DOI：10.3969/j.issn.1007-7146.2025.01.010

Abstract: To investigate the effect of ginkgolide B on the proliferation and apoptosis of human lung adenocarcinoma (A549) cells and the regulatory effect of IκB kinase/nuclear factor κB (IKK/NF-κB) signaling pathway. A549 cells were cultured in vitro and divided into control group (same volume DMSO solvent) and different concentrations (4, 8, 16, 32 μmol/L) of ginkgolide B group. After 24 hours of intervention, cell counting kit-8 (CCK-8) was used to detect cell viability to screen the optimal concentration of ginkgolide B for subsequent experiments. Then A549 cells were divided into control group, ginkgolide B group (16 μmol/L ginkgolide B), positive drug group (0.4 mg/L adriamycin), inhibitor group (16 μmol/L ginkgolide B+10 μmol/L IKK/NF-κB pathway inhibitor BAY11-7082) and activator group (16 μmol/L ginkgolide B+1 μmol/L IKK/NF-κB pathway activator Prostratin), intervention for 24 hours. 5-ethynyl-2′-deoxyuridine (EdU) was used to measure cell proliferation. Hoechst 33258 staining kit was used to detect cell apoptosis. Western blot (WB) was used to detect the expression levels of CyclinD1, Caspase-3 and IKK/NF-κB pathway-related proteins. According to the results of CCK-8 experiment, 16 μmol/L ginkgolide B was selected for subsequent experiments. Compared with the control group, the proliferation rate of A549 cells and the protein expression levels of CyclinD1, p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα in the ginkgolide B group and the positive drug group significantly decreased (P<0.05), and the apoptosis rate and Caspase-3 protein expression level significantly increased (P<0.05). Compared with ginkgolide B group, cell proliferation rate, CyclinD1, p-NF-κB p65/NF-κB p65 and p-IκBα /IκBα ratio significantly decreased in inhibitor group (P<0.05), while apoptosis rate and Caspase-3 protein expression level significantly increased (P<0.05). In the activator group, cell proliferation rate, CyclinD1, p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα ratios significantly increased (P<0.05), while apoptosis rate and Caspase-3 protein expression levels significantly decreased (P<0.05). Ginkgolide B can inhibit the proliferation and induce apoptosis of A549 cells by regulating IKK/NF-κB pathway signaling.
Key words: adenocarcinoma of the lung; ginkgolide B; IκB kinase/nuclear factor-κB signaling pathway; cell proliferation; cell apoptosis
（Acta Laser Biology Sinica, 2025, 34(1): 084-089）